Recently, Professor Yunfeng Lin’s team atthe State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University has published a research paper titled“Design, Fabrication and Applications of Tetrahedral DNA Nanostructure-based Multifunctional Complexes in DrugDelivery and Biomedical Treatment”. Sichuan University is the sole work unit of this research paper. Tao Zhang, a post-doctoral fellow, is the first author and Yunfeng Lin is the corresponding author.
This paper systematically describes the multifunctional delivery system based on tetrahedral DNA nanomaterials and its application in medical field.
“---Owing to their merits, which include natural biocompatibility, structural stability, unsurpassed programmability, ease of internalization and editable functionality, tetrahedral DNA nanostructures show promising potential as an alternative vehicle for drug delivery and biomedical treatment. Here, we describe the design, fabrication, purification, characterization and potential biomedical applications of a self-assembling tetrahedral DNA nanostructure (TDN)–based multifunctional delivery system. First, relying on Watson-Crick base pairing, four single DNA strands form a simple and typical pyramid structure via one hybridization step. Then, the protocol details four different modification approaches, including replacing a short sequence of a single DNA strand by an antisense peptide nucleic acid, appending an aptamer to the vertex, direct incubation with small-molecular-weight drugs such as paclitaxel and wogonin and coating with protective agents such as cationic polymers. These modified TDN-based complexes promote the intracellular uptake and biostability of the delivered molecules, and show promise in the fields of targeted therapy, antibacterial and anticancer treatment and tissue regeneration. The entire duration of assembly and characterization depends on the cargo type and modification method, which takes from 2 h to 3 d.” (Abstract)
The research team of Yunfeng Lin has carried out a series of research on frame nucleic acids over a long period of time. The preliminary results have been published in important journals at home and abroad, such as MaterialsToday、NanoToday、NanoLetters、SignalTransduction and Targeted Therapy、Nano -Micro Letters、and Small, among other important journals.
Article link：https://www.nature.com/articles/s41596-020-0355-z.
